Human HSP-90 (Heat Shock Protein 90) CLIA Kit (HUES01031)

This Sandwich-CLIA assay from Assay Genie is designed for the accurate measurement of HSP-90 (Heat Shock Protein 90) levels in human samples, including serum, plasma, and cell culture supernatants. The kit uses a chemiluminescence detection method and works on a sandwich immunoassay principle in which standards or samples bind to a pre-coated capture antibody, followed by a biotinylated detection antibody and HRP-conjugated streptavidin. A chemiluminescent substrate is then added and the emitted light is read on a luminometer, with the signal proportional to the concentration of the target protein.

Key specifications include an assay time of 3 h 30 min, a sensitivity of 37.5 pg/mL, a detection range of 62.5-4000 pg/mL, and a recovery of 80%-120%. The recommended sample volume is 100 μL. The kit recognises Human HSP-90 in samples, with no significant cross-reactivity or interference between Human HSP-90 and analogues observed; the associated UniProt ID is P08238. Recommended storage conditions are 2-8°C/-20°C.

HSP-90 is a molecular chaperone protein that plays a critical role in protein folding, stability, and regulation of client proteins, and is often upregulated in response to cellular stress, helping maintain cellular homeostasis. Studying HSP-90 levels can provide valuable insights into cellular stress responses, protein quality control mechanisms, and the development of therapeutic strategies for diseases such as cancer, neurodegenerative disorders, and cardiovascular disease. Research areas supported include cancer and signal transduction.