Human HSD17B4 ELISA Kit (HUFI03888)

This quantitative enzyme-linked immunosorbent assay from Assay Genie is configured to measure hydroxysteroid 17-beta dehydrogenase type 4 (HSD17B4), a peroxisomal multifunctional enzyme, in human samples. The target protein, also known as D-bifunctional protein (DBP) or multifunctional protein 2 (MFP-2), catalyzes the second and third reactions of the peroxisomal beta-oxidation pathway for very long chain fatty acids and contributes to steroid and prostaglandin metabolism.

Reagents are provided in a 96 Assays microplate format with a colorimetric readout. The kit is calibrated for serum or plasma samples, supporting research into peroxisomal biogenesis disorders, fatty acid oxidation defects, and metabolic pathway analysis. All work is performed in vitro and is intended for research use only.

Typical workflow includes standard curve generation across the supplied dilution series, sample addition, incubation, washing, and signal development steps detailed in the included protocol. Researchers investigating D-bifunctional protein deficiency, peroxisomal enzyme expression, or the broader role of HSD17B4 in lipid and steroid metabolism will find this assay useful for quantitative measurement.