Human HRH2 (Histamine H2 receptor) ELISA kit (HUFI03420)

The Assay Genie HRH2 ELISA kit enables quantitative measurement of the Histamine H2 Receptor in serum, plasma, and cell lysate samples through a sandwich immunoassay performed on a pre-coated microplate. The assay employs a human-specific capture antibody, limiting cross-reactivity with homologous receptors from other species.

HRH2 is a G protein-coupled receptor expressed in gastric parietal cells, cardiac tissue, mast cells, and lymphocytes. It mediates histamine-driven gastric acid secretion and acts as the pharmacological target of H2 receptor antagonists such as cimetidine, ranitidine, and famotidine. Researchers investigate HRH2 in ulcer biology, immune cell signaling, cardiac rhythm regulation, and inflammatory disease research.

The kit includes a pre-coated microplate, lyophilised standards, biotinylated detection antibody, streptavidin-HRP conjugate, substrate solution, and wash buffer. A 100 uL sample volume is loaded per well, and absorbance is read on a standard microplate reader. Reconstituted reagents should be aliquoted and stored at -20 degrees C to limit freeze-thaw degradation, while unopened plates and buffers remain stable under refrigerated conditions until the printed expiry date.

This kit is intended for research use only and supports academic, pharmaceutical, and clinical laboratories profiling HRH2 expression in human pharmacology and disease-model studies.