Human HPGD ELISA Kit (HUFI03907)

Quantify 15-hydroxyprostaglandin dehydrogenase (HPGD) levels in human samples using this enzyme-linked immunosorbent assay kit from Assay Genie. HPGD is the principal catabolic enzyme responsible for inactivating prostaglandins PGE2 and PGI2, converting them to their 15-keto metabolites, and it has emerged as a biomarker of significant interest in colorectal cancer research, where loss of HPGD expression is associated with adenoma-to-carcinoma progression.

The kit is supplied as a complete microplate package with pre-coated wells, capture and detection reagents, a standard curve of recombinant HPGD, buffers, and substrate solution. Investigators studying parturition timing, inflammatory mediator turnover, NSAID pharmacology, and tumor suppressor pathways apply this quantitative immunoassay to measure circulating HPGD protein in matched case-control cohorts.

The protocol uses standard laboratory plate-reader equipment and produces a quantitative read-out proportional to HPGD concentration in each loaded specimen, supporting statistical comparison across treatment groups, disease stages, or genotype categories. Each kit provides sufficient reagents for a full microplate run, and the included standards allow construction of a calibration curve for interpolation of unknown sample concentrations.