Human HPD ELISA Kit (HUFI03905)

Human 4-hydroxyphenylpyruvate dioxygenase (HPD) is a non-heme iron-dependent enzyme that catalyzes the second step in tyrosine catabolism, converting 4-hydroxyphenylpyruvate into homogentisic acid. Quantification of circulating HPD levels supports research into inborn errors of tyrosine metabolism, including tyrosinemia type III and hawkinsinuria, where mutations in the HPD gene disrupt normal enzymatic activity and lead to elevated substrate accumulation in affected patients.

This Assay Genie immunoassay is configured in a 96 Assays microplate format with an absorbance-based readout for quantitative measurement. The kit is validated for use with human blood-derived specimen types and is suitable for both research and clinical investigation workflows. All reagents are supplied ready-to-use, and unopened components should be kept refrigerated between uses to preserve enzymatic activity and binding performance.

Researchers studying hepatic enzyme expression, inherited metabolic disease, and aromatic amino acid processing will find this kit useful for biomarker validation. The platform supports in vitro assay workflows and accommodates high-throughput measurement, allowing reproducible data generation across larger cohort studies or focused pilot experiments. A detailed protocol is included, outlining recommended dilution series, sample preparation steps, and the standard assay procedure for consistent results.