Human HP-IgG (Helicobcter Pylori-Immunoglobulin G) ELISA Kit (HUFI04713)

This indirect biotin ELISA kit from Assay Genie enables quantitative detection of human IgG antibodies specific to Helicobacter pylori in serum or plasma samples. The microplate-based format supports clinical research into gastritis, peptic ulcer disease, and gastric MALT lymphoma, where serological IgG profiling complements urea breath testing, stool antigen assays, and biopsy-based diagnostic methods. The procedure involves an indirect capture format with biotin-streptavidin amplification, producing a measurable enzymatic endpoint readable on a standard microplate reader. Each plate supports up to 96 determinations using approximately 10 uL of diluted sample per well, with a standard curve calibrated across a low U/mL range for sensitive IgG quantification. The kit is provided for in vitro research use only and is not approved for diagnostic procedures. All reagents should be refrigerated between uses to preserve antibody binding activity, and unused strip wells should be kept dry in the supplied sealed pouch with desiccant. Researchers should avoid repeated freeze-thaw cycles of reconstituted standards and refer to the included manual for complete calibration, dilution, and wash protocol details.