Human HOXB1 ELISA Kit (HUFI03915)

Homeobox B1 (HOXB1) is a developmental transcription factor studied in hindbrain patterning, neuronal specification, and emerging oncology contexts where its expression is profiled alongside other HOX family members. The Assay Genie Human HOXB1 ELISA Kit delivers a colorimetric in vitro immunoassay workflow for quantifying HOXB1 protein levels in human research samples processed under standard cell culture and tissue handling conditions.

Each 96 Assays kit ships with a pre-coated microplate, lyophilized recombinant standards in sealed vials, biotinylated detection antibody, streptavidin-HRP conjugate, TMB substrate, and concentrated wash buffer — sufficient reagents for one full plate run with internal standard curve replicates. Working dilutions and reconstituted reagents should be refrigerated between uses, and the kit is labeled for research use only rather than clinical diagnostic procedures.

Absorbance is read on a standard microplate reader, and a four-parameter logistic fit is recommended for standard curve interpolation. Researchers working with limited biopsy material or rare primary samples can run partial plates by sealing unused wells, since the assay is built around standard laboratory equipment and routine ELISA workflows.