Human HOMER3 ELISA Kit (HUFI07803)

The HOMER3 (Homer Scaffold Protein 3) protein is a neuronal scaffolding molecule of the Homer family, characterized by an N-terminal Enabled/VASP homology 1 (EVH1) domain that recognizes proline-rich motifs and a C-terminal coiled-coil region responsible for self-assembly into tetramers. This Assay Genie kit quantifies HOMER3 in serum and plasma samples using a sandwich immunoassay with colorimetric readout, supporting studies of postsynaptic density regulation, calcium signaling at endoplasmic reticulum-plasma membrane junctions, and cytoskeletal remodeling in neuronal and non-neuronal tissues. The assay includes pre-coated plates, biotinylated detection antibody, standards, and all reagents needed for quantitative measurement; the protocol specifies a 100 µL sample volume with incubation steps performed at room temperature, and unopened components should be refrigerated at 4°C until first use. Researchers apply HOMER3 quantification to investigate neuropsychiatric conditions, cerebellar function, and signaling pathway crosstalk where distinct Homer isoforms play differing roles.