Human HO2 (Heme Oxygenase 2) ELISA Kit (HUES03074)

The Assay Genie Human HO2 (Heme Oxygenase 2) ELISA Kit is a colorimetric sandwich immunoassay configured on a 96-well microplate for the quantitative detection of native Heme Oxygenase 2 in human serum, plasma, and other biological fluids. The assay is calibrated against recombinant human HO-2 standards, with intra-assay and inter-assay precision typically below 10% CV, and exhibits negligible cross-reactivity to the inducible HO-1 isoform, allowing researchers to discriminate constitutive heme catabolism from stress-induced signaling.

HO-2 is a constitutive ~36 kDa enzyme that converts heme into biliverdin, carbon monoxide, and free iron, supporting cellular homeostasis in neurons, vascular endothelium, and reproductive tissues. Investigators studying oxidative stress, neuroprotection, or cardiovascular signaling pathways commonly apply this kit with cell culture supernatants and tissue homogenates. Each kit includes a pre-coated 96-well plate, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer, substrate solution, stop reagent, and recombinant standards sufficient for 96 determinations. Reconstituted reagents should be stored refrigerated at 2-8 degrees C and protected from light, while unused wells can be sealed and kept at 2-8 degrees C for short-term use; for longer storage, freeze-thaw cycles should be minimized to preserve antibody activity.

For research use only; not intended for diagnostic procedures.