Human HNRPA2B1 ELISA Kit

From Assay Genie, this Sandwich ELISA Kit enables quantitative measurement of HNRPA2B1 (Heterogeneous Nuclear Ribonucleoprotein A2/B1) in human samples, validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid sample types. The double-antibody sandwich assay uses colorimetric detection with TMB substrate, read at 450 nm.

Linearity across 1:2, 1:4, and 1:8 dilutions spans 81-105% in serum, EDTA plasma, and heparin plasma. Recovery ranges from 89-105% in serum (average 97%), 85-105% in EDTA plasma (average 93%), and 87-105% in heparin plasma (average 98%).

The standard protocol involves primary incubation at 37°C for 90 minutes, biotinylated detection antibody binding for 60 minutes, HRP-Streptavidin incubation for 30 minutes, and TMB colour development for 10-20 minutes in the dark. Kit components include a pre-coated ELISA microplate, lyophilised standard, biotin-labelled antibody (100X), HRP-Streptavidin conjugate (SABC, 100X), TMB substrate, sample dilution buffer, antibody dilution buffer, SABC dilution buffer, stop solution, wash buffer (25X), plate sealer, and technical manual. The product is stored at 2-8°C for 12 months and is intended for immunology research, supporting studies of RNA metabolism, gene expression, cancer, neurological disorders, and autoimmune conditions.