Human HNRNPUL1 ELISA Kit (HUFI03928)

HNRNPUL1 (Heterogeneous Nuclear Ribonucleoprotein U-Like 1) is a nuclear RNA-binding protein involved in mRNA processing, transcript stability, and the DNA damage response, and it has attracted attention as a candidate biomarker in hepatocellular carcinoma and other oncology research. This immunoassay from Assay Genie is supplied as a complete kit including pre-coated microplate wells, biotinylated detection antibody, recombinant protein standards, streptavidin-HRP conjugate, wash buffer, and substrate solution, sufficient for 96 Assays. Researchers typically apply it to liquid biopsies, tissue lysates, and cell culture supernatants to quantify endogenous HNRNPUL1 levels, loading 100 µL of sample per well in duplicate. Standard curves are generated from serially diluted reference antigen, allowing interpolation of unknown sample concentrations across the linear working range. Unopened reagents should be kept refrigerated at 4°C with the microplate sealed in its foil pouch; reconstituted standards and diluted detection antibody are best aliquoted and stored frozen to limit freeze-thaw cycles and preserve activity. The protocol supports research use only applications including biomarker discovery, pathway profiling, and comparative analysis between diseased and healthy donor cohorts, with each run completed through absorbance readout following the final substrate incubation.