Human HMGCS2 (Hydroxymethylglutaryl-CoA synthase, mitochondrial) ELISA KIT (HUFI08155)

The Assay Genie HMGCS2 ELISA kit supports quantitative measurement of mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase in human specimens. HMGCS2 is the rate-limiting enzyme of hepatic ketogenesis, catalyzing the condensation of acetyl-CoA with acetoacetyl-CoA to form hydroxymethylglutaryl-CoA, a precursor of acetoacetate and beta-hydroxybutyrate. Expression rises sharply during fasting, prolonged exercise, and adherence to ketogenic diets. Investigators in metabolic disease, type 2 diabetes, hepatic biology, and mitochondrial disorders rely on this assay to track pathway activation, examine the consequences of loss-of-function variants associated with hypoketotic hypoglycemia, and probe responses to pharmacological PPAR-alpha agonists. The microplate format includes pre-coated capture antibodies, detection antibody, standards, and substrate reagents sufficient for a complete run. Reconstituted components should be stored refrigerated with longer-term storage below freezing; protect from light and limit freeze-thaw cycles of working aliquots. Intended for research use only.