Human HMGCL ELISA Kit (HUFI03925)

Quantify 3-Hydroxy-3-Methylglutaryl-CoA Lyase (HMGCL), the mitochondrial enzyme that catalyzes the final step of ketogenesis, with this plate-based immunoassay from Assay Genie. HMGCL converts HMG-CoA into acetoacetate and acetyl-CoA and also closes the leucine catabolic pathway, so reliable measurement of its protein levels is relevant to metabolic disease research, mitochondrial dysfunction studies, and investigations of hypoketotic hypoglycemia and fatty acid oxidation disorders.

The kit is configured for research use with human-derived specimens such as serum samples or heparin plasma, supporting clinical research laboratories and in vitro studies of ketone body metabolism. Each kit provides reagents sufficient for 96 determinations in a microplate format, supporting both small-scale pilot work and larger cohort screens.

Researchers studying enzyme expression in hepatic tissue, the pathogenesis of organic acidurias, or the regulation of ketogenesis under varying nutritional states will find this kit suited to those workflows. Standard laboratory ELISA equipment and a plate reader capable of measuring absorbance are sufficient for the readout.

The assay is intended for research use only, not for diagnostic procedures. Store reagents as indicated on the product insert to maintain stability across the shelf life of the kit.