Human HMGB3/High mobility group protein B3 ELISA Kit

From Assay Genie, this Sandwich ELISA Kit is specifically designed for the accurate and reliable detection of HMGB3 (High Mobility Group Protein B3) levels in human samples. It is intended for research applications and uses a double antibody format with a colorimetric readout.

The assay is reactive with human samples, including serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Reported sensitivity is 0.188 ng/mL and the working range is 0.313–20 ng/mL. Linearity across 1:2, 1:4, and 1:8 dilutions is reported as 90–103% / 94–98% / 96–100% for serum, 83–100% / 83–100% / 83–97% for EDTA plasma, and 83–90% / 87–98% / 85–96% for heparin plasma (n = 5). Recovery is reported as 86–96% (average 90%) for serum, 85–101% (average 95%) for EDTA plasma, and 85–104% (average 94%) for heparin plasma. Storage is 2–8°C for 12 months.

HMGB3 is a member of the high mobility group protein family and has been implicated in cellular processes including DNA repair, transcriptional regulation, and inflammation, with dysregulation linked to cancer, autoimmune disorders, and inflammatory conditions. The kit targets the O15347 UniProt entry and falls within the immunology research area.