Human HMG box-containing protein 1 (HBP1) ELISA Kit

Manufactured by Assay Genie, this Sandwich ELISA is designed for the precise quantitative measurement of HMG box-containing protein 1 (HBP1) in human serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. The assay is intended for research use only and supports investigations into cancer biology, where HBP1 — a nuclear transcriptional repressor — contributes to the regulation of the cell cycle and the Wnt pathway.

Key performance specifications include a detection range of 0.156–10 ng/mL and a sensitivity of 0.082 ng/mL. Precision is characterised by an intra-assay CV of 4.8% and an inter-assay CV of 8.2%. Linearity has been demonstrated across serial dilutions in serum (102–121%), EDTA plasma (95–117%) and heparin plasma (88–115%) sample matrices. Recovery averages 93% in serum (range 87–99%) and 95% in plasma (range 89–101%).

The kit detects both natural and recombinant human HBP1 (UniProt O60381) and is suited to studies exploring gene expression regulation, cell differentiation, apoptosis and tumorigenesis. Researchers are advised to refer to the kit's component insert for exact storage conditions and protocol details.