Human HLCS ELISA Kit (HUFI03943)

Quantitative measurement of human holocarboxylase synthetase (HLCS) protein levels with this colorimetric ELISA from Assay Genie, intended for research use only. HLCS is the biotin-protein ligase responsible for covalently attaching biotin to multiple mitochondrial carboxylases, including pyruvate carboxylase, acetyl-CoA carboxylase, propionyl-CoA carboxylase, and 3-methylcrotonyl-CoA carboxylase, making it a relevant target for studies of biotin metabolism, inherited multiple carboxylase deficiency, and broader metabolic disease research. The kit is supplied in a configuration sufficient for 96 Assays and supports laboratory workflows investigating enzyme expression in patient-derived material or relevant cell line models. A standard curve-based workflow enables comparison of HLCS levels across experimental conditions, and the included protocol and reagents are formatted for routine immunoassay workflows in academic and translational research settings.