Human HLA-F ELISA Kit (HUFI03952)

The Human HLA-F ELISA Kit from Assay Genie enables quantitative measurement of HLA-F, a non-classical MHC class I heavy chain implicated in immune tolerance, NK cell regulation, and maternal-fetal interface biology. The assay is specific to human HLA-F, and cross-reactivity with rodent MHC-Ib orthologues has not been observed, supporting transplantation immunology, reproductive biology, and viral immune evasion studies in clinical cohorts.

Each kit contains a pre-coated microplate, detection antibodies, standard calibrators, and buffers sufficient for 96 reactions, with reagents requiring refrigerated storage between uses. The standard curve spans 0.156–10 ng/mL with a minimum detectable dose of approximately 0.05 ng/mL. The protocol fits routine immunology workflows and supports research use only investigations into MHC-Ib family function. Detection relies on absorbance readings from a standard plate reader, and the kit is intended for laboratory research settings.