Human HLA class II histocompatibility antigen, DR beta 5 chain (HLA-DRB5) ELISA Kit

Assay Genie provides a competitive ELISA kit for the quantitative detection of human HLA class II histocompatibility antigen, DR beta 5 chain (HLA-DRB5). The kit is validated for use with human serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids, and is supplied for research use only.

Performance specifications include a detection range of 1.56-100 ng/mL and a sensitivity of 0.16 ng/mL. Reported precision values are an intra-assay CV of 6.3% and an inter-assay CV of 9.4%. Recovery is documented at an average of 100% (range 94-106%) for serum and 102% (range 96-108%) for plasma. Specificity is stated for the natural and recombinant form of human HLA class II histocompatibility antigen, DR beta 5 chain (UniProt Q30154).

Biologically, HLA-DRB5 contributes to immune function by binding peptides derived from antigens that access the endocytic route of antigen-presenting cells and presenting them on the cell surface for recognition by CD4 T-cells, with the peptide binding cleft accommodating peptides of 10-30 residues. The kit is positioned for research in immunology, autoimmune diseases, infectious diseases, and inflammatory conditions.