Human HLA class I (HLA-B) ELISA Kit (HUFI03941)

HLA class I (HLA-B) is a cell-surface glycoprotein encoded within the major histocompatibility complex that presents intracellular peptide antigens to CD8+ cytotoxic T lymphocytes, making quantitative measurement of its soluble form a recurring need in transplantation immunology, autoimmune disease research, and population genetics studies. This in vitro research immunoassay from Assay Genie is supplied in a microplate format sufficient for 96 individual determinations and uses a colorimetric readout to quantify HLA-B in human serum or plasma specimens. The kit includes pre-coated plate wells, biotinylated detection antibody, standard diluent, substrate solution, concentrated wash buffer, and stop reagent. Researchers commonly deploy this assay when profiling soluble HLA levels in transplant recipients, screening patient cohorts for HLA-B27 or HLA-B57 alleles associated with ankylosing spondylitis or HIV progression respectively, or characterizing HLA shedding by tumor cell lines in vitro. All reagents carry a research use only designation and should be stored refrigerated between uses to preserve antibody binding activity across the kit's working shelf life.