Human Histone H4 (HIST1H4A) ELISA Kit

Designed for the precise measurement of Histone H4 (Hist1H4A) levels in human samples, this sandwich ELISA from Assay Genie is intended for research applications. The kit is compatible with serum, plasma, tissue homogenates, cell culture supernates and other biological fluids, and uses a colorimetric detection method. It specifically recognises natural and recombinant human Histone H4.

According to the manufacturer's specifications, the kit delivers a detection range of 0.156-10ng/mL with a sensitivity of 0.078ng/mL. Reported precision values include an intra-assay CV of 4.2% and an inter-assay CV of 8.1%. Linearity has been evaluated across serial dilutions in serum, EDTA plasma and heparin plasma, with recovery averaging 88% (range 82-94%) in serum and 90% (range 84-96%) in plasma.

Histone H4 is a core component of the nucleosome, playing a central role in chromatin structure, transcription regulation, DNA repair and DNA replication. The kit is classified under the epigenetics research area, with the target localised to the nucleus and chromosome. Synonyms H4/A and H4FA correspond to the same target, and the UniProt reference is P62805. The kit is for research use only.