Human Histone H1 (H1) ELISA Kit

The Human Histone H1 ELISA Kit from Assay Genie enables quantitative measurement of the linker histone H1, a key component of higher-order chromatin structure responsible for nucleosome spacing and DNA compaction. Researchers apply this kit to evaluate H1 levels across multiple sample matrices, supporting studies in epigenetics, chromatin remodeling, and cellular stress responses.

Working with a standard microplate format, the assay provides quantitative readouts on a conventional plate reader. Unopened kits are shipped refrigerated and stored at 4 °C; reconstituted reagents and standards are typically held frozen at -20 °C in single-use aliquots to limit freeze-thaw cycles. Each well accommodates up to 100 µL of sample, and overnight incubation protocols are available for low-abundance chromatin extracts.

The kit is intended for research use only and employs human-specific capture antibodies, with no significant cross-reactivity reported against related histone variants, supporting clean detection in nuclear lysates and chromatin preparations.