Human HIST3H3 (Histone H3.1t) ELISA Kit (HUFI03433)

Researchers investigating chromatin biology, epigenetic regulation, and male germ cell development need reliable quantitative tools to measure specific histone variants in human specimens. This Assay Genie ELISA Kit enables sensitive detection of HIST3H3, also known as Histone H3.1t, a replication-independent member of the histone H3 family that is preferentially expressed during spermatogenesis and incorporated into nucleosomes in developing germ cells. The immunoassay workflow supports translational research into histone variant switching, nucleosome assembly dynamics, and infertility-related chromatin remodeling, giving laboratories a reproducible way to compare HIST3H3 levels across experimental groups. The kit is supplied as a complete 96-assay reagent set, including pre-coated microplate strips, calibrated recombinant standards, capture and detection antibodies, wash buffer, and chromogenic substrate components required to generate a measurable, concentration-dependent signal. Each kit is intended for research use only and integrates with standard laboratory workflows that rely on microplate-based absorbance reading and routine ELISA data analysis.