Human HISPPD1 ELISA Kit (HUFI03946)

The Human HISPPD1 ELISA Kit from Assay Genie enables quantitative measurement of the human HISPPD1 protein in clinical research samples using a microplate-based immunoassay format. The kit includes a pre-coated strip plate, lyophilized recombinant protein standards, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer, substrate solution, and stop reagent sufficient for 96 individual determinations. Researchers apply this assay to biomarker validation studies, translational projects, and investigation of neurological or metabolic pathways where this protein has been implicated. The colorimetric readout is captured on a standard microplate reader, and the kit is supplied with a detailed protocol describing sample preparation, standard curve generation, and result calculation. Unopened kits should be stored refrigerated at 4 degrees C and protected from light, with the reconstituted standard aliquoted and stored frozen at -20 degrees C to limit freeze-thaw cycles. Components are packaged in individual vials within a sealed kit box, allowing single-use reagent preparation where needed. The assay uses a human-specific capture antibody, validated for detection of endogenous human HISPPD1 with no significant cross-reactivity observed against closely related human phosphatase-domain proteins, and is intended for research use only, providing reproducible quantification of endogenous HISPPD1 levels without significant interference from matrix components in properly prepared samples.