Human his (Histamine) ELISA Kit (HUFI04788)

Built on a competitive immunoassay platform, this Assay Genie kit enables quantitative measurement of endogenous histamine in clinical research samples, supporting allergy, mast cell activation, and inflammatory response studies. Histamine (2-[4-imidazolyl]ethylamine), a low-molecular-weight biogenic amine derived from histidine decarboxylation, is captured via antibody-coated wells using a competitive format appropriate for small-molecule antigens. Each microplate provides 96 assays with colorimetric readout, and the protocol uses a 50 uL sample volume per well. The kit is supplied with reagents for in vitro research use only and should be refrigerated at 4 degrees C until first use. Researchers in immunology, pulmonology, and gastroenterology commonly apply this kit to monitor histamine release in translational studies.