Human HIF-1 alpha ELISA Kit

This immunoassay kit from Assay Genie is a sandwich ELISA using a double antibody format for the in vitro quantitative determination of human HIF1A concentrations in serum, plasma and other biological fluids. Reactivity is human, and the documented sample types include serum, plasma, tissue homogenates, cell lysates, urine, cerebrospinal fluid, cell culture supernatant and breast milk. The kit is for research use only and is stored at 4°C for 6 months.

Recovery for spiked samples was measured in serum (range 89-105%, average 98%), EDTA plasma (87-94%, average 91%) and UFH plasma (88-103%, average 96%), with linearity demonstrated at 1:2, 1:4 and 1:8 dilutions. Intra-assay CV is

HIF1A (Hypoxia Inducible Factor 1 Subunit Alpha) is a master transcriptional regulator of the adaptive response to hypoxia, activating the transcription of over 40 genes under hypoxic conditions, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor and HILPDA. The associated UniProt entry is Q16665, NCBI Gene ID 3091, with a molecular weight of 95,634 Da.