Human HGF PharmaGenie ELISA Kit (SBRS0671)

This ELISA kit from Assay Genie quantifies Hepatocyte Growth Factor (HGF) in human research specimens. HGF drives hepatocyte proliferation, epithelial–mesenchymal signaling through the c-MET receptor, angiogenesis, and tissue regeneration, making it a frequent biomarker in oncology, liver injury, wound healing, and stem-cell differentiation studies. The kit accommodates a broad sample portfolio, supporting plasma-derived and serum-derived fractions, cell culture supernatants, cell lysates, and tissue lysates from human origins.

Each kit provides reagents sufficient for 96 determinations in a microplate-based workflow. A standard curve is generated from recombinant HGF calibrators, and bound analyte is revealed with an enzyme-labeled detection antibody followed by a colorimetric substrate reaction. Absorbance is read on a standard plate reader, and results are interpolated against the included standard curve.

Storage conditions call for refrigeration of all components until first use, with frozen aliquots maintained at the supplier-recommended temperature and protected from light. Reconstituted standards and working-strength detection antibody should be used promptly and are not intended for repeated freeze-thaw cycles; aliquoting after reconstitution is recommended. The kit is supplied for research use only and is not cleared for diagnostic procedures.

Applications include pathway analysis of HGF/MET signaling in cancer cell lines, monitoring of angiogenic factor release in endothelial cell culture, and quantitative profiling of circulating HGF in disease-cohort research.