Human Hexokinase-3 (HK3) ELISA Kit

From Assay Genie, this Sandwich ELISA Kit is intended for the quantitative measurement of human Hexokinase-3 (HK3), also referred to as Hexokinase type III, Hexokinase-C, or HK III, in serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. The colorimetric assay is suited to research investigating hexokinase 3 levels in human samples, supporting a range of studies into metabolic pathways.

Hexokinase 3 is a glycolytic enzyme that catalyses the first and rate-limiting step of glycolysis by phosphorylating D-glucose to D-glucose 6-phosphate. As an allosteric enzyme, it is inhibited by its product glucose-6-phosphate and acts as a "glucose sensor" by trapping glucose inside the cell. Dysregulation of hexokinase 3 has been linked to diabetes, cancer and neurodegenerative disorders, underlining its value as a potential biomarker and therapeutic target. The corresponding protein is listed under UniProt accession P52790.

The kit reports a detection range of 0.156-10ng/mL and a sensitivity of 0.076ng/mL. Reported precision includes an intra-assay CV of 5.6% and an inter-assay CV of 9.9%. Specificity covers natural and recombinant human Hexokinase-3. The product is for research use only.