Human Heterogeneous Nuclear Ribonucleoprotein K (HNRPK) ELISA Kit

Quantify HNRPK Levels in Human Research Samples

Heterogeneous Nuclear Ribonucleoprotein K (HNRPK), also known as hnRNP K, is a multifunctional RNA-binding protein with K-homology (KH) domains that mediates transcription, pre-mRNA splicing, translational silencing, and chromatin remodeling. Altered HNRPK abundance has been documented in colorectal, hepatocellular, and breast cancers, and the protein participates in multiple signaling cascades including p53, WNT, and c-MYC pathways, making it a target of interest for oncology, RNA biology, and signal transduction research.

This Assay Genie ELISA kit delivers a quantitative immunoassay engineered for the human protein. It is configured as a single-use 96-assay plate pack and is intended for research use only, in vitro measurements. Validated sample inputs include tissue homogenate preparations and cell lysates, so investigators can assess endogenous HNRPK expression across cultured cells, xenograft-derived material, and primary tissue specimens without cross-species adaptation.

Typical applications include gene expression profiling, biomarker validation, mechanistic studies of post-transcriptional regulation, and screening of compound libraries for modulators of RNA-binding protein activity. The well plate layout supports high-throughput workflows in academic laboratories, biotech R&D teams, and shared core facilities.

Each kit ships with pre-coated microplate wells, matched detection antibody, standards, and buffers for running the assay. Consult the enclosed product manual for detailed protocol steps, recommended sample dilution ranges, and plate-reader setup guidance specific to this lot.