Human Hephaestin ELISA Kit

Designed by Assay Genie for research applications, this sandwich ELISA enables precise quantification of hephaestin levels in human samples. Hephaestin plays a key role in iron metabolism, contributing to iron transport and absorption, and its dysregulation has been linked to disorders such as hemochromatosis and anemia, making accurate monitoring valuable for studying these conditions and related iron metabolism research.

The kit is a double-antibody sandwich ELISA with a sensitivity of 46.875 pg/mL and a measurable range of 78.125–5000 pg/mL. It is intended for use with human serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, with reagents stored at 2–8°C for up to 12 months. Reported performance characteristics include linearity of 83–104% and recovery of 85–104% across serum, EDTA plasma, and heparin plasma samples. The workflow uses a biotin-labeled detection antibody, an HRP-streptavidin conjugate (SABC), and a TMB substrate, with absorbance read at 450 nm after addition of stop solution.

A typical run involves equilibrating reagents and substrate to room temperature, loading standards, samples and a zero control onto the pre-coated plate, incubating at 37°C for antigen binding, applying the biotinylated detection antibody, adding the SABC reagent, developing colour in the dark for 10–20 minutes, and reading absorbance immediately after the stop reaction. Target synonyms include HEPH and Hephaestin (UniProt Q9BQS7), and the kit is intended for Cell Biology research.