Human Hepcidin ELISA Kit

From Assay Genie, this sandwich ELISA kit is designed for the precise detection of Hepcidin in human serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Using a double antibody format with colorimetric detection, the assay delivers high sensitivity and specificity to support accurate and reproducible quantification across a range of research applications.

Hepcidin is a key regulator of iron metabolism, controlling the absorption and distribution of iron in the body. Dysregulation of hepcidin has been associated with conditions such as anemia, hemochromatosis, and chronic kidney disease. The kit achieves a sensitivity of 18.75 pg/mL with an assay range of 31.25-2000 pg/mL.

Linearity across 1:2 to 1:8 dilutions spans 86-102% for serum, 83-99% for EDTA plasma, and 80-104% for heparin plasma. Recovery averages 94% for serum, 92% for EDTA plasma, and 93% for heparin plasma. Reagents should be stored at 2-8°C for 12 months. The kit targets human Hepcidin (Uniprot P81172), used in cell biology research.