Human Heparin Cofactor II (HCII) ELISA Kit

HCII (Heparin Cofactor II), a serine protease inhibitor also known as SERPIND1, plays a distinct role in hemostasis by neutralizing thrombin activity, particularly when bound to dermatan sulfate. This immunoassay from Assay Genie enables researchers to quantify circulating HCII concentrations in human plasma, supporting studies of venous thromboembolism, thrombotic risk, and congenital SERPIND1 deficiency. The plate is pre-coated with a capture antibody specific to human HCII, and detection relies on an HRP-conjugated secondary reagent read on a standard microplate reader. Reconstituted standards and biotinylated detection antibody should be stored at -20 °C after receipt, while working dilutions may be kept refrigerated for short-term use. Each kit includes a pre-coated microplate, lyophilized standards, detection reagent, wash buffer, and substrate solution. Researchers investigating coagulation cascades, fibrinolysis, or serpin biology will find the kit useful for clinical plasma analysis and mechanistic studies of thrombin regulation.