Human Heme Oxygenase 2, Decycling (HO2) ELISA Kit

Quantifying the constitutive human heme oxygenase 2 (HO-2) isoform is the purpose of this Assay Genie ELISA Kit. HO-2 catalyzes the degradation of heme into biliverdin, free iron, and carbon monoxide, serving distinct physiological roles from the stress-inducible HO-1 counterpart in neuronal, endothelial, and hepatic tissues. Research teams studying oxidative stress, neurovascular signaling, and inflammatory regulation routinely employ HO-2 quantification to investigate baseline and challenged cellular states.

Sample compatibility includes tissue homogenates, cell lysates, and cell culture supernatants, supporting workflows across cardiovascular, neuroscience, and toxicology laboratories. Reagents are supplied in vials packaged for 96 assays, with components to generate a standard curve alongside experimental replicates on a microplate reader. The included protocol walks through plate setup, incubation, and readout steps using an absorbance-based measurement approach.

Unopened kits should be refrigerated and protected from light to preserve antibody and standard performance across multiple experimental runs. Each kit ships with sufficient material for the full plate layout and is intended for research use only, providing laboratories with a reproducible pathway to assess HO-2 protein levels in vitro.