Human HELB ELISA Kit (HUFI07814)

DNA Helicase B (HELB) is a 5'-to-3' single-stranded DNA-dependent helicase with central roles in the replication stress response, the ATR-CHK1 checkpoint, and homology-directed DNA repair. This quantitative immunoassay from Assay Genie enables measurement of HELB in human serum or plasma samples, supporting translational research into chemoresistance, replication fork protection, and tumor cell survival under genotoxic stress.

Built around a pre-coated microplate format supplied as a 96 Assays kit with a colorimetric readout, the assay is configured for cohort-scale screening on standard laboratory plate readers. Each run follows a wash-and-read workflow intended for research use only, with reagents that integrate directly into existing DNA damage response and immunology pipelines.

HELB is recruited to RPA-coated single-stranded DNA at stalled forks and modulates downstream checkpoint signaling, so quantitative profiling helps researchers track replication fork collapse, evaluate the cellular response to replication-blocking lesions, and screen for biomarkers linked to oncogenesis and therapy resistance. The Assay Genie kit is validated for human reactivity and serves studies spanning cancer biology, helicase biochemistry, and the cellular response to DNA-damaging agents.