Human Hedgehog Interacting Protein (HHIP) ELISA Kit

Quantitative measurement of Human Hedgehog Interacting Protein (HHIP) in cell lysates, tissue homogenates and other human research samples is supported by the colorimetric ELISA Kit produced by Assay Genie. HHIP acts as a membrane-bound negative regulator of the Hedgehog signaling pathway, binding Sonic, Indian and Desert hedgehog ligands and modulating their activity during embryonic patterning, stem cell maintenance and adult tissue homeostasis. Altered HHIP expression has been documented in chronic obstructive pulmonary disease research, lung cancer studies, pancreatic adenocarcinoma investigations and hepatocellular carcinoma models, positioning the protein as a target of interest in oncology, developmental biology and respiratory disease laboratories.

Each Assay Genie kit is configured to run 96 assays per plate using a precoated microplate format, and the assay is reactive specifically with human HHIP. The kit is intended for research use only, fitting laboratories running cell culture, western blot follow-up, immunohistochemistry and gene expression studies on Hedgehog pathway components. Validated sample types include tissue homogenates and cell lysates, supporting workflows that begin with cultured cell lines, primary tissue specimens or animal model material.

Reagents are supplied ready to use or as concentrated stocks depending on the component, and the kit should be protected from light during storage to preserve reagent activity. Researchers should refer to the lot-specific certificate of analysis included with the shipment for working dilution, recommended sample volume and standard curve reconstitution details.