Human HEBP2 ELISA Kit (HUFI03967)

Heme Binding Protein 2 (HEBP2) is a small intracellular heme-binding protein also known as p22HBP or SOUL. This Assay Genie immunoassay kit enables quantitative measurement of HEBP2 in human serum and EDTA plasma specimens, supporting research into iron homeostasis, mitochondrial heme trafficking, and oxidative stress responses. Each kit supplies a pre-coated microplate, detection reagents, recombinant standards, conjugate, substrate, wash buffer, and protocol documentation sufficient for 96 determinations. Absorbance is recorded on a standard plate reader, and results are calculated against the included standard curve to determine sample HEBP2 concentrations. Working reagents should be stored at 4 °C between uses, with the reconstituted standard kept frozen in single-use aliquots to preserve activity across the stated shelf life. Researchers in cell biology, hematology, and metabolic disease laboratories apply the kit to studies of heme metabolism, mitochondrial iron-sulfur cluster biogenesis, and erythroid differentiation pathways. The assay is labeled for research use only and is not intended for clinical diagnostic decision-making. Reproducibility is supported by intra-assay and inter-assay precision data provided with each lot, allowing laboratories to generate consistent quantitative outputs for downstream Western blot, PCR, or immunohistochemistry workflows.