Human Heat Shock Protein Beta 6 (HSPb6) ELISA Kit

Additional assay characteristics for the Assay Genie HSPb6 kit include specificity for both natural and recombinant human HSPB6, with intra-assay precision reported at CV <8% and inter-assay precision at CV <10% across three concentration levels. The immunopair detects the 17,136 Da monomer (UniProt O14558; NCBI Gene ID 126393; chromosomal locus 19q13.12), which assembles into homodimers and higher-order oligomers that exchange subunits dynamically. Subcellular distribution spans cytoplasm, nucleus, and secreted pools, with documented translocation into nuclear foci during heat shock.

The protein carries alternative designations HEL55 and PPP1R91, and mechanistically stabilizes monomeric 14-3-3ζ (YWHAZ) to sustain its chaperone-like activity. Its overexpression has been linked to cardioprotection, angiogenesis through KDR signaling, and modulation of cardiac myocyte contractility. Researchers studying chaperone-mediated proteostasis, smooth muscle contractility, or post-ischemic remodeling can pair this ELISA with Western blot or immunohistochemistry workflows to confirm expression across cardiac, vascular, and skeletal tissue lysates. Reconstituted standard should be aliquoted and frozen at -20 °C to limit freeze-thaw cycles, and the TMB substrate equilibrated at room temperature for at least 30 minutes before use. For research use only.