Human Heat Shock 70kDa Protein 5 (HSPA5) ELISA Kit

The HSPA5 enzyme-linked immunosorbent assay quantifies a 78 kDa endoplasmic reticulum chaperone that doubles as a canonical biomarker of unfolded protein response activation. Because BiP/GRP78 expression rises sharply when misfolded proteins accumulate, researchers deploy this quantitative immunoassay to track ER stress in cell culture models, primary tissue homogenates, and patient-derived biospecimens. The kit supports translational work in oncology, where elevated HSPA5 correlates with tumor aggressiveness and chemoresistance, as well as investigations into neurodegeneration, metabolic disease, and viral host-cell interactions. Assay Genie supplies the reagents in a 96-assay microplate configuration with absorbance-based detection, allowing batch processing on standard plate readers. Compatible human matrices encompass clarified sera, plasma-derived fractions, solubilized tissue preparations, whole-cell lysates, and clarified cell culture media, with each run requiring only a small microliter-scale aliquot of clarified sample. The protocol pairs straightforward incubation steps with a single wavelength measurement, making it practical for high-throughput screening of stress-modulating compounds, genetic perturbations, or clinical cohorts. Because GRP78 is also secreted under chronic stress, the assay can monitor circulating biomarker levels in longitudinal studies. Reagents should be kept refrigerated and protected from light until first use, with the microplate sealed between runs to preserve antibody activity.