Human HDLBP/Vigilin ELISA Kit

From Assay Genie, this sandwich ELISA kit enables quantitative measurement of HDLBP/Vigilin in human samples using a colorimetric detection method. HDLBP/Vigilin is involved in RNA metabolism and transport and in the regulation of gene expression, and its dysregulation has been linked to cancer, neurodegenerative disorders, and cardiovascular diseases, making it a useful research biomarker [S1].

Reported sensitivity is 0.938 ng/mL with a working range of 1.563-100 ng/mL [S1]. Validated sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples [S1]. The kit should be stored at 2-8°C for 12 months [S1]. Linearity assessed at 1:2, 1:4, and 1:8 dilutions (n=5) spanned 82-101% for serum, 85-105% for EDTA plasma, and 80-105% for heparin plasma across dilutions [S1]. Average recovery was 91% for serum, 96% for EDTA plasma, and 92% for heparin plasma [S1].

The protocol follows a sandwich ELISA workflow: reagent and plate equilibration, primary incubation at 37°C, biotinylated detection antibody binding, HRP-streptavidin incubation, TMB substrate development in the dark for 10-20 minutes, and absorbance measurement at 450 nm [S1]. Sample preparation guidance is provided for serum, plasma, tissue homogenate, cell culture supernatant, and cell lysate [S1].