Human HDC (Histidine decarboxylase) ELISA KIT (HUFI05155)

This quantitative immunoassay kit from Assay Genie is configured for measuring human histidine decarboxylase (HDC), the pyridoxal-5'-phosphate-dependent enzyme that catalyzes the conversion of L-histidine to histamine. HDC expression is a focal point in research on mast cell biology, gastric enterochromaffin-like cell function, allergic inflammation, and certain neuroendocrine neoplasms, making reliable enzyme quantitation valuable for studies exploring histamine biosynthesis regulation.

The kit supports research workflows that require sensitive detection of HDC in human-derived specimens. Beyond standard use with circulating blood fractions, the assay has been applied to cell culture supernatants and tissue homogenate preparations, extending its utility to in vitro cell-based models of mast cell activation and to rodent tissue studies where cross-tissue HDC activity is profiled. The format includes a precoated microplate, biotinylated detection antibody, calibrated reference standards, and streptavidin-HRP reagents.

Researchers investigating gastrointestinal histamine signaling, mastocytosis biomarkers, or the intersection of immune and neuroendocrine pathways frequently pair this kit with downstream applications including western blot confirmation, immunohistochemistry of gastric or cutaneous biopsies, and flow cytometry characterization of mast cell populations. Unopened components should be refrigerated and protected from light to preserve reagent activity. Intended for research use only.