Human HDAC4 (Histone deacetylase 4) ELISA Kit (HUFI03972)

Quantitative measurement of human Histone Deacetylase 4 (HDAC4) protein in biological samples supports research into chromatin remodeling, gene expression regulation, and a range of disease pathways. HDAC4 is a class IIa histone deacetylase involved in transcriptional repression through interaction with transcription factors such as MEF2, and altered HDAC4 abundance has been studied in the context of cardiac hypertrophy, skeletal muscle differentiation, neuronal stress responses, and several tumor types including breast and prostate cancer.

This Assay Genie kit is configured as a 96 Assays format, providing enough microplate wells and reagents to run full standard curves alongside experimental samples in a single batch. The assay is intended for in vitro research use only and is applied to study HDAC4 levels in human specimen types relevant to investigations of epigenetic regulation and disease biomarkers.

Samples should be handled according to standard immunoassay practice: collect specimens using appropriate anticoagulants, separate by centrifugation, aliquot to avoid repeated freeze-thaw cycles, and store refrigerated at 4°C for short-term use or frozen at -80°C for longer archival storage. Reagent components should be brought to room temperature before use, and the assay workflow incorporates incubation, washing, and detection steps compatible with standard microplate readers.

Researchers studying HDAC4 signaling, histone modification pathways, or screening compounds that modulate class IIa deacetylase activity will find this ELISA useful for quantifying protein levels and comparing expression across patient cohorts, treated versus untreated cell cultures, or wild-type versus knockout animal models. As a research-use-only tool, it is suitable for academic laboratories, pharmaceutical target validation, and biomarker discovery projects.