Human HCN4 ELISA Kit

Manufactured by Assay Genie, this Sandwich ELISA (Double Antibody) is designed for the quantitative measurement of hyperpolarization-activated cyclic nucleotide-gated channel 4 (HCN4) in human samples. Validated sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The kit is intended for signal transduction research and is relevant to studies of cardiac pacemaker activity, arrhythmias, and heart failure.

Reported performance figures are a sensitivity of 0.188 ng/mL and a working range of 0.313–20 ng/mL. Dilutional linearity at 1:2, 1:4, and 1:8 falls within 81–104% across serum, EDTA plasma, and heparin plasma, while average recovery is 90% (serum), 94% (EDTA plasma), and 97% (heparin plasma). Components are stored at 2–8°C, with the lyophilized standard stable for up to 12 months at ‒20°C. The workflow involves a 90-minute primary incubation at 37°C, a biotinylated detection antibody, HRP-streptavidin binding, and TMB substrate read at 450 nm.