Human HCN3 ELISA Kit (HUFI03981)

Quantitative profiling of HCN3 (Hyperpolarization-activated Cyclic Nucleotide-gated channel 3) protein in human specimens is enabled by this colorimetric immunoassay from Assay Genie. HCN3 is one of four HCN channel isoforms that carry the hyperpolarization-activated "funny" current (If/Ih) governing pacemaker activity in sinoatrial node cardiomyocytes and shaping rhythmic firing in selected neuronal populations. Aberrant HCN3 expression has been reported in studies of cardiac arrhythmia, sinoatrial node dysfunction, and respiratory rhythm control, so protein-level tracking complements electrophysiology and transcript-level readouts in cardiovascular and neuroscience research workflows.

Shipped as a 96 Assays kit, the assay is supplied as a complete microplate configuration with pre-coated wells, lyophilized standards, detection reagents, and substrate solution sufficient for one full plate run on a standard plate reader. Components arrive in a refrigerated pack and should be stored refrigerated between runs; reconstituted standards and working detection antibody are aliquoted to limit freeze-thaw cycles and preserve binding activity over the working shelf life of the reagents.

Intended for research use only, this HCN3 ELISA supports laboratories building ion channel biomarker panels in heart failure tissue samples, sinoatrial node disease models, neuronal pacemaker studies, or screens evaluating small-molecule modulators of HCN channel expression. Each kit includes a detailed protocol insert from Assay Genie outlining plate layout, wash procedure, and standard curve generation for accurate interpolation of unknown sample concentrations in the assay run.