Human HAI-1 PharmaGenie ELISA Kit (SBRS0658)

Hepatocyte Growth Factor Activator Inhibitor-1 (HAI-1), encoded by the SPINT1 gene, is a transmembrane Kunitz-type serine protease inhibitor that restrains HGFA and matriptase, the two principal activators of the HGF/MET signaling pathway. The Assay Genie HAI-1 PharmaGenie ELISA Kit enables researchers to quantify endogenous HAI-1 levels in human cell culture supernatants, plasma, and serum using a 96-assay microplate format with a colorimetric readout suitable for standard absorbance plate readers.

Each kit ships as a complete workflow package, including pre-coated microplate wells, biotinylated detection antibody, recombinant human HAI-1 standard, streptavidin-HRP conjugate, wash buffer, substrate, and stop reagent, supporting straightforward assay setup. Intended for research use only, the kit serves laboratories investigating epithelial barrier biology, placental and reproductive biology, hepatocyte injury, and tumor invasion where dysregulated pericellular proteolysis and HGF/MET signaling are implicated.

Because HAI-1 shedding and surface expression shift in response to stimuli such as cellular stress, cytokine exposure, and metastatic transition, accurate quantification of the soluble ectodomain supports biomarker discovery, mechanistic studies, and target validation workflows in oncology, hepatology, and inflammation research.