Human H3F3B ELISA Kit (HUFI03989)

This ELISA kit from Assay Genie enables quantitative measurement of H3F3B, the gene encoding the replication-independent histone variant H3.3B, in clinical research samples. H3F3B deposits H3.3 at active gene bodies, telomeric regions, and sites of DNA damage, supporting transcription-coupled chromatin remodeling outside of S-phase. Researchers investigating pediatric high-grade gliomas, giant cell tumors of bone, chondroblastomas, and other neoplasms characterized by H3.3 hotspot alterations can use this assay to probe protein abundance in patient biofluids. Each kit includes a pre-coated microplate, capture and detection antibodies, calibrated standards, chromogenic substrate, wash buffer, and stop solution, providing enough reagent for one plate run. Store components refrigerated between uses and protect from light; minimize freeze-thaw cycles of the antibody vials and reconstituted standard. The plate reader measures absorbance after the enzymatic color reaction develops, and a standard curve is generated from a serial dilution of the supplied reference antigen.