Human GYG1 ELISA Kit (HUFI03985)

Glycogenin-1 is the autocatalytic glucosyltransferase that primes glycogen biosynthesis by attaching the first glucose residue to itself, after which glycogen synthase and the branching enzyme elongate the polymer. The Assay Genie Human GYG1 ELISA Kit provides a quantitative immunoassay for measuring this priming enzyme in human research specimens, supporting work on skeletal muscle glycogen turnover, polyglucosan body myopathy (glycogen storage disease type XV, linked to recessively inherited GYG1 mutations), cardiomyocyte glucose handling, and metabolic changes in insulin-resistant states. The kit is supplied as a 96 Assays plate for research use only, and produces a colorimetric read-out compatible with standard absorbance microplate readers. Validated human specimen sources include serum-based collections and EDTA plasma; researchers typically pair the assay with parallel tissue mRNA or Western blot analysis of GYG1 expression, single-use aliquots stored under freeze-thaw control, and a standard curve generated per the enclosed protocol. Working reagents, detection conjugate, wash buffer, and plate seals are all included in the box, and reconstituted components should be stored refrigerated between runs.