Human Guanylate Cyclase Activator 2B/GUCA2B ELISA Kit

Assay Genie offers this Sandwich ELISA kit for the quantitative detection of human Guanylate Cyclase Activator 2B (GUCA2B) in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The assay uses a colorimetric detection method in a double-antibody sandwich format, supporting research into guanylate cyclase activation and signaling pathways.

GUCA2B is described as a critical regulator of guanylate cyclase activity, with roles in smooth muscle relaxation and water-electrolyte balance; its dysregulation has been linked to gastrointestinal disorders and hypertension. Reported performance characteristics include a sensitivity of 9.375 pg/mL, an assay range of 15.625-1000 pg/mL, and storage at 2-8°C for 12 months. Spike-and-recovery averaged 96% in serum (range 85-104%), 97% in EDTA plasma (86-105%), and 90% in heparin plasma (85-103%). Linearity at 1:2 to 1:8 dilutions spanned 81-105% across these matrices.

The workflow is a six-step protocol: equilibrate reagents and TMB substrate and load the pre-coated plate; incubate at 37°C for 90 minutes; add biotinylated detection antibody and incubate for 60 minutes; add HRP-Streptavidin (SABC) for 30 minutes; develop with TMB substrate in the dark for 10-20 minutes; then add stop solution and read absorbance at 450 nm.