Human GTF2H2 ELISA Kit (HUFI04013)

The GTF2H2 protein (General Transcription Factor IIH subunit 2) is a core component of the TFIIH complex, where it contributes to RNA polymerase II-driven transcription initiation and the nucleotide excision repair pathway that clears bulky DNA lesions from the genome. This research-use immunoassay from Assay Genie is configured to quantify GTF2H2 levels in human samples and is supplied as a 96-test microplate kit.

Investigators studying the DNA damage response, transcription-coupled repair, and the molecular biology of cancer cell lines commonly track GTF2H2 abundance to probe how cells cope with genotoxic stress, how transcriptional programs shift during differentiation, and how inherited variants in TFIIH subunits give rise to disease phenotypes such as xeroderma pigmentosum, Cockayne syndrome, and trichothiodystrophy. The kit uses a matched-pair antibody design and an HRP-TMB chromogenic reaction that is read on a standard microplate reader. Reconstitution of the lyophilized standard and the incubation schedule follow a sequential, batch-friendly workflow that accommodates serum or plasma alongside cell lysate and tissue homogenate.

Kit contents typically include a pre-coated microplate, biotinylated detection antibody, recombinant standard, streptavidin-HRP conjugate, wash buffer, substrate solution, and stop reagent. Reagents should be stored refrigerated, with working aliquots of standard and conjugate held frozen to limit activity loss across repeated freeze-thaw cycles. Lot-specific dynamic range, lower limit of detection, and intra- and inter-assay precision values are detailed in the certificate of analysis supplied with each shipment.