Human GTF2F1 ELISA Kit (HUFI04022)

The GTF2F1 gene encodes General Transcription Factor IIF Subunit 1, also called RAP74, a protein that functions within the general transcription factor IIF complex essential for RNA polymerase II-driven mRNA transcription initiation. This Assay Genie ELISA Kit enables quantitative measurement of human GTF2F1 protein levels through a colorimetric readout and is configured for 96 Assays. The kit is intended for research use only and supports studies of transcriptional regulation, RNA polymerase II activity, and pre-initiation complex assembly in human-derived samples.

Investigators studying transcription factor expression, gene regulation, and the molecular machinery of mRNA synthesis apply this ELISA kit to profile GTF2F1 abundance across cell culture models, primary cells, and tissue homogenates. Each kit provides the reagents required for a complete ELISA workflow, including antibody-coated microplate wells. The assay accepts small sample volumes, which is helpful when specimen material is limited. To preserve reagent integrity, store refrigerated and protect from light exposure, which maintains antibody binding activity and substrate performance across the shelf life of the product.

Beyond baseline quantification, the kit supports comparative studies, such as measuring GTF2F1 changes in response to drug treatment, siRNA knockdown, or differentiation stimuli, and it complements orthogonal techniques like western blot and qPCR in transcription factor research programs.