Human GTF2A1/Transcription initiation factor TFIIA 42 kDa subunit/TFIIA-42/General transcription factor IIA subunit 1/TFIIAL ELISA Kit

Assay Genie's sandwich ELISA kit enables colorimetric detection of GTF2A1 (General Transcription Factor IIA Subunit 1) in human samples. Using a double antibody sandwich format, the assay is validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, and is intended for research in epigenetics and nuclear signaling.

The kit delivers a sensitivity of 0.188 ng/mL with a working range of 0.313-20 ng/mL. The protocol follows a structured workflow: equilibrate reagents and TMB substrate to room temperature, perform a 90-minute primary incubation at 37 °C, bind the biotin-labelled detection antibody for 60 minutes, add HRP-Streptavidin (SABC) for 30 minutes, develop with TMB substrate in the dark for 10–20 minutes, then stop the reaction and read absorbance at 450 nm.

Validated linearity and recovery support reliable quantitation: linearity ranges from 80% to 105% across serum, EDTA plasma, and heparin plasma at 1:2 to 1:8 dilutions, while average recovery falls between 93% and 97% across the same matrices. Storage is specified at 2-8 °C for 12 months.