Human GTF2A1 (General Transcription Factor IIA, Polypeptide 1) ELISA Kit (HUES01618)

The Assay Genie sandwich ELISA also supports mechanistic studies of RNA polymerase II transcription initiation, including analysis of TBP stabilization at TATA-containing promoters, chromatin remodeling dynamics, and co-activator recruitment. The TFIIA-α/β subunit interacts directly with TBP and contributes to transcriptional activation across multiple gene classes, enabling assessment of chromatin changes under differentiation, hormone treatment, or cellular stress.

Typical assays complete in approximately 3–4 hours, with detection sensitivity in the low pg/mL range. Recommended sample volumes are 50–100 µL per well, and intra-assay precision generally falls below 10% CV. Comparable TFIIA immunoassays have been applied in hepatocellular carcinoma, acute myeloid leukemia, and other malignancies characterized by dysregulated Pol II activity, correlating protein abundance with transcriptional output. Limit freeze-thaw cycles of reconstituted reagents to preserve performance. For research use only.